ps_choice
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ps_choice [27/11/2017 13:59] pieter created |
ps_choice [20/08/2019 07:12] (current) pieter [Technique] |
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| |WESTERN BLOTTING|_|_|_|_|_|_|✔|_|_|_|_| | |WESTERN BLOTTING|_|_|_|_|_|_|✔|_|_|_|_| | ||
| |SEMI-DRY BLOTTING|_|_|_|_|_|_|✔|_|_|_|_| | |SEMI-DRY BLOTTING|_|_|_|_|_|_|✔|_|_|_|_| | ||
| - | |MINI WESTERN BLOTTING|_|_|✔|_|_|_|_|_|_|_|_| | + | |MINI WESTERN BLOTTING|_|_|✔|_|_|_|✔|_|_|_|_| |
| - | |MINI SEMI-DRY BLOTTING|_|_|✔|_|_|_|_|_|_|_|_| | + | |MINI SEMI-DRY BLOTTING|_|_|✔|_|_|_|✔|_|_|_|_| |
| + | ==== Voltage/ | ||
| + | Each device has certain maximum limits for Voltage, Current and Power. Depending on the technique, the choice of the **maximum Voltage** is important, it determines the strength of the electrical field. The choice is also determined by the **size of the gel/tank** that will be connected. \\ | ||
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| + | The maximum current is important especially for blotting techniques. It is also important when required to connect multiple tanks to the same power supply. It is recommended to connect tanks of the same size and requiring the same Voltage when using only one power supply for them. Each tank requires a certain **amount of current and power** (law of Ohm, see further). This amount has to be multiplied with the amount of tanks that are to be connected. This way is it possible that the device limits of the current or the power are tresspassed and prevent a correct execution of the process. It is recommended to check the power supplies maximum limits in comparison to the required voltage, current and power for the technique, tank size and **amount of tanks**. | ||
ps_choice.1511791144.txt.gz · Last modified: 27/11/2017 13:59 by pieter
